ARG65529
anti-TCR alpha + beta antibody [R73]
anti-TCR alpha + beta antibody [R73] for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Rat,Primates
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [R73] recognizes TCR alpha + beta |
|---|---|
| Tested Reactivity | Rat, NHuPrm |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | The clone R73 recognizes TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | R73 |
| Isotype | IgG1 |
| Target Name | TCR alpha + beta |
| Immunogen | Rat T blasts and erythrocytes |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | Functional studies: In vitro activation and in vivo depletion of TCR alpha/beta T cells. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression._x000D_ |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | TCR alpha: 30 kDa TCR beta: 35 kDa |
Images (1) Click the Picture to Zoom In
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ARG65529 anti-TCR alpha + beta antibody [R73] FACS image
Flow Cytometry: Rat thymocyte suspension stained with ARG65529 anti-TCR alpha + beta antibody [R73] at 1.6 μg/ml dilution.
