ARG63436
anti-TCP1 antibody
anti-TCP1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes TCP1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TCP1 |
| Antigen Species | Human |
| Immunogen | C-SYEDAVHSGALND |
| Conjugation | Un-conjugated |
| Alternate Names | T-complex protein 1 subunit alpha; CCT1; D6S230E; TCP-1-alpha; CCTa; CCT-alpha |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 60 kDa |
Images (4) Click the Picture to Zoom In
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ARG63436 anti-TCP1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63436 anti-TCP1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63436 anti-TCP1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Testis. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63436 anti-TCP1 antibody at 10 µg/ml dilution followed by HRP-staining.
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ARG63436 anti-TCP1 antibody WB image
Western blot: 35 µg of Human ovary (A) and HEK293 (B) lysates (in RIPA buffer) stained with ARG63436 anti-TCP1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63436 anti-TCP1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63436 anti-TCP1 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
