ARG22256
anti-TCP1 alpha antibody [91a]
anti-TCP1 alpha antibody [91a] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,C. elegans,D. melanogaster ,Dog,Guinea pig,Hamster,Monkey,Pig,Rabbit,Yeast
Overview
| Product Description | Rat Monoclonal antibody [91a] recognizes TCP1 alpha |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Ce, Dm, Dog, Gpig, Hm, Mk, Pig, Rb, Yeast |
| Tested Application | FACS, ICC/IF, IP, WB |
| Specificity | Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 91a |
| Isotype | IgG2a |
| Target Name | TCP1 alpha |
| Antigen Species | Mouse |
| Immunogen | Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region). |
| Conjugation | Un-conjugated |
| Alternate Names | T-complex protein 1 subunit alpha; CCT1; D6S230E; TCP-1-alpha; CCTa; CCT-alpha |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | ~60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol |
| Preservative | 0.1% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Tcp1 |
| Gene Full Name | t-complex protein 1 |
| Background | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010] |
| Function | Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. [UniProt] |
| Cellular Localization | Cytoplasm |
| Calculated MW | 60 kDa |
Images (3) Click the Picture to Zoom In
-
ARG22256 anti-TCP1 alpha antibody [91a] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22256 anti-TCP1 alpha antibody [91a] (yellow) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
-
ARG22256 anti-TCP1 alpha antibody [91a] WB image
Western blot: 1) MW ladder, 20 µg of 2) A431 lysate, and 3) HEK293 lysate stained with ARG22256 anti-TCP1 alpha antibody [91a] (60 min at RT). Block: 5% milk + TBST 1hr at RT.
-
ARG22256 anti-TCP1 alpha antibody [91a] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22256 anti-TCP1 alpha antibody [91a] (red) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
