ARG66629
anti-Synaptophysin antibody [SQab19147]
anti-Synaptophysin antibody [SQab19147] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab19147] recognizes Synaptophysin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab19147 |
| Isotype | IgG |
| Target Name | Synaptophysin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 213-313 of Human Synaptophysin. |
| Conjugation | Un-conjugated |
| Alternate Names | Synaptophysin; MRXSYP; Major synaptic vesicle protein p38; MRX96 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SYP |
| Gene Full Name | synaptophysin |
| Background | This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011] |
| Function | Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity). [UniProt] |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome. [UniProt] |
| Calculated MW | 34 kDa |
| PTM | Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66629 anti-Synaptophysin antibody [SQab19147] WB image
Western blot: 20 μg of Mouse brain and Rat brain lysates stained with ARG66629 anti-Synaptophysin antibody [SQab19147] at 1:500 dilution.
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ARG66629 anti-Synaptophysin antibody [SQab19147] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human glioma tissue stained with ARG66629 anti-Synaptophysin antibody [SQab19147]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
Customer's Feedback
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Review for anti-Synaptophysin antibody [SQab19147]
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
