ARG56230

anti-Synaptophysin antibody

anti-Synaptophysin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Synaptophysin
Tested Reactivity Hu, Ms
Predict Reactivity Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Synaptophysin
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 225-253 (C-terminus) of Human Synaptophysin.
Conjugation Un-conjugated
Alternate Names Synaptophysin; MRXSYP; Major synaptic vesicle protein p38; MRX96

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:100
IHC-P1:1000
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse cerebellum

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20977 Mouse SYP

GeneID: 6855 Human SYP

Swiss-port # P08247 Human Synaptophysin

Swiss-port # Q62277 Mouse Synaptophysin

Gene Symbol SYP
Gene Full Name synaptophysin
Background This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity). [UniProt]
Cellular Localization Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome
Calculated MW 34 kDa
PTM Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

Images (4) Click the Picture to Zoom In

  • ARG56230 anti-Synaptophysin antibody ICC/IF image

    Immunofluorescence: Human brain cells stained with ARG56230 anti-Synaptophysin antibody (green). DAPI (blue) for nuclear staining.

  • ARG56230 anti-Synaptophysin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue stained with ARG56230 anti-Synaptophysin antibody.

  • ARG56230 anti-Synaptophysin antibody WB image

    Western blot: 35 µg of Mouse cerebellum lysate stained with ARG56230 anti-Synaptophysin antibody.

  • ARG56230 anti-Synaptophysin antibody FACS image

    Flow Cytometry: Neuro-2a cells stained with ARG56230 anti-Synaptophysin antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.