ARG63795

anti-Syk antibody

anti-Syk antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody; SyK / Zap70 Pathway antibody

Overview

Product Description Goat Polyclonal antibody recognizes Syk
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application WB
Specificity This antibody is expected to recognise both reported isoforms (NP_003168.2; NP_001128524.1). Reported pairs of variants represent identical proteins (NP_003168.2 and NP_001167638.1; NP_001128524.1 and NP_001167639.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Syk
Antigen Species Human
Immunogen C-KYLQQNRHVKDKN
Conjugation Un-conjugated
Alternate Names Tyrosine-protein kinase SYK; p72-Syk; Spleen tyrosine kinase; EC 2.7.10.2

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6850 Human SYK

Swiss-port # P43405 Human Tyrosine-protein kinase SYK

Background This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody; SyK / Zap70 Pathway antibody
Calculated MW 72 kDa
PTM Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation.
Autophosphorylated. Phosphorylated on tyrosine residues by LYN following receptors engagement. Phosphorylation on Tyr-323 creates a binding site for CBL, an adapter protein that serves as a negative regulator of BCR-stimulated calcium ion signaling. Phosphorylation at Tyr-348 creates a binding site for VAV1. Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3-kinase-independent pathway (By similarity). Phosphorylation on Ser-297 is very common, it peaks 5 minutes after BCR stimulation, and creates a binding site for YWHAG. Phosphorylation at Tyr-630 creates a binding site for BLNK. Dephosphorylated by PTPN6.

Images (1) Click the Picture to Zoom In

  • ARG63795 anti-Syk antibody WB image

    Western blot: Human Spleen lysate (35 µg protein in RIPA buffer) stained with ARG63795 anti-Syk antibody at 1 µg/ml dilution.