ARG51724
anti-Stathmin 1 phospho (Ser16) antibody
anti-Stathmin 1 phospho (Ser16) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Stathmin 1 phospho (Ser16) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Stathmin 1 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 16 (R-A-S(p)-G-Q) derived from Human Stathmin 1. |
| Conjugation | Un-conjugated |
| Alternate Names | PP17; Prosolin; Stathmin; Protein Pr22; PR22; Lag; C1orf215; PP19; pp19; SMN; OP18; Leukemia-associated phosphoprotein p18; LAP18; pp17; Oncoprotein 18; Phosphoprotein p19; Op18; Metablastin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | STMN1 |
| Gene Full Name | stathmin 1 |
| Background | Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear |
| Function | Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity). [UniProt] |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 17 kDa |
| PTM | Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. |
Images (3) Click the Picture to Zoom In
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ARG51724 anti-Stathmin 1 phospho (Ser16) antibody WB image
Western blot: Extracts from 293 cells untreated or treated with Nocodazol stained with ARG51724 anti-Stathmin 1 phospho (Ser16) antibody.
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ARG51724 anti-Stathmin 1 phospho (Ser16) antibody WB image
Western blot: Extracts from HeLa cells, treated with Noc or calf intestinal phosphatase (CIP), stained with ARG51724 anti-Stathmin 1 phospho (Ser16) antibody.
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ARG51724 anti-Stathmin 1 phospho (Ser16) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51724 anti-Stathmin 1 phospho (Ser16) antibody (left) or the same antibody preincubated with blocking peptide (right).
