ARG54040
anti-Smad 5 antibody (C-term)
anti-Smad 5 antibody (C-term) for Flow cytometry,ICC/IF,Western blot and Human
Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes Smad 5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | Smad 5 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | DWFC; Smad5; JV5-1; Mothers against decapentaplegic homolog 5; Mothers against DPP homolog 5; hSmad5; MADH5; SMAD 5; MAD homolog 5; SMAD family member 5 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99717 Human Mothers against decapentaplegic homolog 5 |
|---|---|
| Gene Symbol | SMAD5 |
| Gene Full Name | SMAD family member 5 |
| Background | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus |
| Research Area | Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 52 kDa |
| PTM | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. |
Images (3) Click the Picture to Zoom In
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ARG54040 anti-Smad 5 antibody (C-term) ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54040 anti-Smad 5 antibody (C-term) at 1:75 dilution. Left: primary antibody (green). Right: DAPI (blue).
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54040 anti-Smad 5 antibody (C-term) WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG54040 anti-Smad 5 antibody (C-term) at 1:1000 dilution.
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ARG54040 anti-Smad 5 antibody (C-term) FACS image
Flow Cytometry: Jurkat cells stained with ARG54040 anti-Smad 5 antibody (C-term) at 1:100 dilution (right histogram) or isotype control (left histogram), followed by incubation with FITC labelled secondary antibody.
Customer's Feedback
Excellent
Review for anti-Smad 5 antibody (C-term)
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:75
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
Review for anti-Smad 5 antibody (C-term)
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:75
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Good
Review for anti-Smad 5 antibody (C-term)
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
