ARG11042
anti-Siglec 8 antibody [7C9]
anti-Siglec 8 antibody [7C9] for ELISA,Flow cytometry,Immunoprecipitation and Human
Overview
| Product Description | Mouse Monoclonal antibody [7C9] recognizes Siglec 8 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7C9 |
| Isotype | IgG1 |
| Target Name | Siglec 8 |
| Antigen Species | Human |
| Immunogen | SIGLEC8-Fc protein, containing entire extracellular region of Human Siglec-8 fused with the Fc region of Human IgG1. |
| Conjugation | Un-conjugated |
| Alternate Names | Siglec-8; Sialic acid-binding Ig-like lectin 8; SIGLEC-8; SAF-2; Sialoadhesin family member 2; SAF2; SIGLEC8L |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | SIGLEC8 transfected CHO cells. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NYZ4 Human Sialic acid-binding Ig-like lectin 8 |
|---|---|
| Gene Symbol | SIGLEC8 |
| Gene Full Name | sialic acid binding Ig-like lectin 8 |
| Background | Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010] |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein [UniProt] |
| Calculated MW | 54 kDa |
