ARG63804
anti-Serotonin Transporter antibody
anti-Serotonin Transporter antibody for ICC/IF,Western blot and Human,Rat
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Publication1
Overview
| Product Description | Goat Polyclonal antibody recognizes Serotonin Transporter |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms |
| Tested Application | ICC/IF, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Serotonin Transporter |
| Antigen Species | Human |
| Immunogen | C-LHQGERETWGK |
| Conjugation | Un-conjugated |
| Alternate Names | SERT; 5HTT; SERT1; Sodium-dependent serotonin transporter; hSERT; 5-HTTLPR; Solute carrier family 6 member 4; HTT; 5-HTT; 5HT transporter; OCD1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P31645 Human Sodium-dependent serotonin transporter Swiss-port # P31652 Rat Sodium-dependent serotonin transporter |
|---|---|
| Background | This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma. [provided by RefSeq, Jul 2008] |
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 70 kDa |
| PTM | Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms. Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation. Phosphorylation upon PKC stimulation modifies the SERT distribution and density in the membrane, and diminishes the uptake capacity. |
Images (2) Click the Picture to Zoom In
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ARG63804 anti-Serotonin Transporter antibody WB image
Western blot: Human Colon lysate (35 µg protein in RIPA buffer) stained with ARG63804 anti-Serotonin Transporter antibody at 2 µg/ml dilution.
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ARG63804 anti-Serotonin Transporter antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63804 anti-Serotonin Transporter antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
Specific References
Fecal Microbiota Transplantation Can Alleviate Gastrointestinal Transit in Rats with High Fat Diet-Induced Obesity Via Regulation of Serotonin Biosynthesis
WB / Rat
