ARG64000
anti-Semaphorin 5A antibody
anti-Semaphorin 5A antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Semaphorin 5A |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P |
| Specificity | The immunizing peptide corresponds to a part of the extracellular domain. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Semaphorin 5A |
| Antigen Species | Human |
| Immunogen | DEATKKACYSKGKS |
| Conjugation | Un-conjugated |
| Alternate Names | semF; Semaphorin-5A; Sema F; SEMAF; Semaphorin-F |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010] |
| Research Area | Neuroscience antibody |
| Calculated MW | 121 kDa |
Images (1) Click the Picture to Zoom In
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ARG64000 anti-Semaphorin 5A antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64000 anti-Semaphorin 5A antibody at 4 µg/ml dilution followed by HRP-staining.
