ARG63722
anti-Semaphorin 3E antibody
anti-Semaphorin 3E antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Semaphorin 3E |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Semaphorin 3E |
| Antigen Species | Human |
| Immunogen | C-KPEHYRLPRHTLDS |
| Conjugation | Un-conjugated |
| Alternate Names | Semaphorin-3E; SEMAH; M-SemaK; coll-5; M-SEMAH |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
| Research Area | Neuroscience antibody |
| Calculated MW | 89 kDa |
Images (1) Click the Picture to Zoom In
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ARG63722 anti-Semaphorin 3E antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Adrenal Gland. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63722 anti-Semaphorin 3E antibody at 2.5 µg/ml dilution followed by AP-staining.
