ARG45164

anti-Semaphorin 3B antibody

anti-Semaphorin 3B antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Semaphorin 3B
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Semaphorin 3B
Antigen Species Human
Immunogen Recombinant protein containing to human Semaphorin 3B.
Conjugation Un-conjugated
Alternate Names SEMA3B; semaphorin 3B; SEMA3B; Semaphorin-3B; Sema A (V); Semaphorin-V; Sema V; SEMA3B; SEMA5; SEMAA

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF2 μg/ml
IHC-P0.5-1 μg/ml
WB0.1-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 83 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose.
Preservative 0.01% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7869 Human SEMA3B

Swiss-port # Q13214 Human Semaphorin-3B

Gene Symbol SEMA3B
Gene Full Name semaphorin 3B
Background The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Function Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.. [UniProt]
Cellular Localization Endoplasmic reticulum; Secreted. [UniProt]
Calculated MW 83 kDa
PTM Disulfide bond; Glycoprotein. [UniProt]

Images (7) Click the Picture to Zoom In

  • ARG45164 anti-Semaphorin 3B antibody IHC-P image

    Immunohistochemistry: Human mammary cancer stained with ARG45164 anti-Semaphorin 3B antibody at 1 μg/ml dilution.

  • ARG45164 anti-Semaphorin 3B antibody ICC/IF image

    Immunofluorescence: A431 stained with ARG45164 anti-Semaphorin 3B antibody at 2 ug/ml dilution.

  • ARG45164 anti-Semaphorin 3B antibody WB image

    Western blot: Human placenta, U2OS, and U-87MG stained with ARG45164 anti-Semaphorin 3B antibody at 0.5 μg/ml dilution.

  • ARG45164 anti-Semaphorin 3B antibody IHC-P image

    Immunohistochemistry: Rat brain stained with ARG45164 anti-Semaphorin 3B antibody at 1 μg/ml dilution.

  • ARG45164 anti-Semaphorin 3B antibody WB image

    Western blot: Rat thymus and rat kidney stained with ARG45164 anti-Semaphorin 3B antibody at 0.5 μg/ml dilution.

  • ARG45164 anti-Semaphorin 3B antibody IHC-P image

    Immunohistochemistry: Mouse brain stained with ARG45164 anti-Semaphorin 3B antibody at 1 μg/ml dilution.

  • ARG45164 anti-Semaphorin 3B antibody WB image

    Western blot: Mouse thymus, mouse lung, mouse small intestine, mouse kidney, and HEPA1-6 stained with ARG45164 anti-Semaphorin 3B antibody at 0.5 μg/ml dilution.