ARG66521
anti-Sall4 antibody
anti-Sall4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes Sall4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b, kappa |
| Target Name | Sall4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human Sall4. |
| Conjugation | Un-conjugated |
| Alternate Names | dJ1112F19.1; Zinc finger protein 797; DRRS; Zinc finger protein SALL4; Sal-like protein 4; HSAL4; ZNF797 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Tris/EDTA buffer (pH 8.0) was used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 130 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SALL4 |
| Gene Full Name | spalt-like transcription factor 4 |
| Background | The protein encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS). [provided by RefSeq, Jul 2008] |
| Function | Transcription factor with a key role in the maintenance and self-renewal of embryonic and hematopoietic stem cells. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 112 kDa |
| PTM | Isoform SALL4B exists primarily as a ubiquitinated form. Sumoylation with both SUMO1 and SUMO2 regulates the stability, subcellular localization, transcriptional activity, and may reduce interaction with POU5F1/OCT4. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG66521 anti-Sall4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human seminoma (high magnification) stained with ARG66521 anti-Sall4 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Tris/EDTA buffer (pH 8.0) was used.
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ARG66521 anti-Sall4 antibody WB image
Western blot: 30 µg of HeLa and HEK293 whole cell lysates stained with ARG66521 anti-Sall4 antibody at 1:1000 dilution.
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ARG66521 anti-Sall4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human seminoma stained with ARG66521 anti-Sall4 antibody at 1:200 (4°C, overnight). Antigen Retrieval: Tris/EDTA buffer (pH 8.0) was used.
