ARG63197
anti-SWAP70 antibody
anti-SWAP70 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SWAP70 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SWAP70 |
| Antigen Species | Human |
| Immunogen | C-EEREKNWKEKKTTE |
| Conjugation | Un-conjugated |
| Alternate Names | SWAP-70; Switch-associated protein 70; HSPC321 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SWAP70 |
| Gene Full Name | SWAP switching B-cell complex 70kDa subunit |
| Function | Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion (By similarity). [UniProt] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 69 kDa |
| PTM | Tyrosine-phosphorylated. |
Images (2) Click the Picture to Zoom In
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ARG63197 anti-SWAP70 antibody WB image
Western Blot: Human Lymph Node lysate (35 µg protein in RIPA buffer) stained with ARG63197 anti-SWAP70 antibody at 0.1 µg/ml dilution.
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ARG63197 anti-SWAP70 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63197 anti-SWAP70 antibody at 5 µg/ml dilution followed by AP-staining.
