ARG22616
anti-SUR1 / ABCC8 antibody [S289-16]
anti-SUR1 / ABCC8 antibody [S289-16] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [S289-16] recognizes SUR1 / ABCC8 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Predict Reactivity | Hu, Hm |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | Does not cross-react with SUR2B. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S289-16 |
| Isotype | IgG1 |
| Target Name | SUR1 / ABCC8 |
| Antigen Species | Rat |
| Immunogen | Fusion protein around aa. 1548-1582 (cytoplasmic C-terminus) of Rat SUR1 / ABCC8. |
| Conjugation | Un-conjugated |
| Alternate Names | TNDM2; Sulfonylurea receptor 1; ABC36; HHF1; PHHI; ATP-binding cassette sub-family C member 8; MRP8; HI; SUR; SUR1; HRINS; SUR1delta2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q09429 Rat ATP-binding cassette sub-family C member 8 |
|---|---|
| Gene Symbol | Abcc8 |
| Gene Full Name | ATP-binding cassette, subfamily C (CFTR/MRP), member 8 |
| Background | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013] |
| Function | Subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K(+) channels and insulin release. [UniProt] |
| Calculated MW | 177 kDa (Rat) |
Images (2) Click the Picture to Zoom In
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ARG22616 anti-SUR1 / ABCC8 antibody [S289-16] ICC/IF image
Immunofluorescence: SK-N-BE cells fixed by 4% Formaldehyde for 15 min at RT. Cells were stained with ARG22616 anti-SUR1 / ABCC8 antibody [S289-16] at 1:100 dilution for 60 min at RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Primary antibody (green). (D) Composite.
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ARG22616 anti-SUR1 / ABCC8 antibody [S289-16] WB image
Western blot: 15 µg of Rat brain membrane lysate stained with ARG22616 anti-SUR1 / ABCC8 antibody [S289-16] at 1:200 for 16 hours at 4°C. Block: 2% BSA and 2% Skim Milk in 1X TBST.
