ARG57103
anti-SUMO2 + SUMO3 antibody [10F1]
anti-SUMO2 + SUMO3 antibody [10F1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [10F1] recognizes SUMO2 + SUMO3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 10F1 |
| Isotype | IgG2b, kappa |
| Target Name | SUMO2 + SUMO3 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-93 of Human SUMO2 / SUMO3. |
| Conjugation | Un-conjugated |
| Alternate Names | SMT3 homolog 2; HSMT3; SMT3B; SUMO-2; Small ubiquitin-related modifier 2; Sentrin-2; SUMO3; SUMO-3; SMT3H2; Ubiquitin-like protein SMT3B; Smt3B; Smt3A |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P61956 Human Small ubiquitin-related modifier 2 |
|---|---|
| Gene Symbol | SUMO2 |
| Gene Full Name | small ubiquitin-like modifier 2 |
| Background | This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. Plays a role in the regulation of sumoylation status of SETX. [UniProt] |
| Calculated MW | 11 kDa |
| PTM | Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4. Cleavage of precursor form by SENP1 or SENP2 is necessary for function. Monoubiquitinated N-terminally by UBE2W, which primes it for RNF4-dependent polyubiquitination by the UBE2V1-UBE2N heterodimer. |
Images (6) Click the Picture to Zoom In
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ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] at 1:500 (Red).
Hoechst 3342 (Blue) for nucleus staining.
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ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] FACS image
Flow Cytometry: Jurkat cell line stained with ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
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ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] IHC-P image
Immunohistochemistry: Paraffin-embedded sections of colorectal cancer tissue stained with ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] at 1:50 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
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ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] WB image
Western blot: 40 µg of 1) HeLa, 2) Jurkat, and 3) K562 cell lysates stained with ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] at 1:1000.
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ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] WB image
Western blot: 40 µg of HL-60 cell lysate stained with ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] at 1:1000.
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ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] WB image
Western blot: 10 ng of 1) SUMO1, 2) SUMO2, and 3) SUMO3 recombinant proteins stained with ARG57103 anti-SUMO2 + SUMO3 antibody [10F1] at 1:1000.
