ARG63584
anti-STUB1 / CHIP antibody
anti-STUB1 / CHIP antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes STUB1 / CHIP |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | STUB1 / CHIP |
| Antigen Species | Human |
| Immunogen | C-DAFISENGWVEDY |
| Conjugation | Un-conjugated |
| Alternate Names | NY-CO-7; Antigen NY-CO-7; UBOX1; EC 6.3.2.-; E3 ubiquitin-protein ligase CHIP; SDCCAG7; CHIP; STIP1 homology and U box-containing protein 1; HSPABP2; SCAR16; Carboxy terminus of Hsp70-interacting protein; CLL-associated antigen KW-8 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | STUB1, or CHIP, is a ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (Min et al., 2008 [PubMed 18411298]).[supplied by OMIM, Jul 2009] |
| Research Area | Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 35 kDa |
| PTM | Monoubiquitinated at Lys-2 following cell stress by UBE2W, promoting the interaction with ATXN3 (By similarity). Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. |
Images (2) Click the Picture to Zoom In
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ARG63584 anti-STUB1 / CHIP antibody WB image
Western Blot: Human Muscle lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63584 anti-STUB1 / CHIP antibody at 0.3 µg/ml dilution.
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ARG63584 anti-STUB1 / CHIP antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63584 anti-STUB1 / CHIP antibody at 2.5 µg/ml dilution followed by AP-staining.
