ARG54297
anti-STRO1 antibody [STRO-1] (PE)
anti-STRO1 antibody [STRO-1] (PE) for Flow cytometry and Human
Controls and Markers antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [STRO-1] recognizes STRO1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The clone STRO-1 recognizes the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | STRO-1 |
| Isotype | IgM |
| Target Name | STRO1 |
| Antigen Species | Human |
| Immunogen | Human CD34 positive bone marrow cells |
| Conjugation | PE |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Buffer | TBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells. |
|---|---|
| Research Area | Controls and Markers antibody |
Images (1) Click the Picture to Zoom In
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ARG54297 anti-STRO1 antibody [STRO-1] (PE) FACS image
Flow Cytometry: Human peripheral blood stained with ARG54297 anti-STRO1 antibody [STRO-1] (PE) and anti-CD45 antibody (Pacific Blue).
