ARG59264
anti-STIP1 antibody
anti-STIP1 antibody for Flow cytometry,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes STIP1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Bov, Hm, Mk |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | STIP1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 461-488 (C-terminus) of Human STIP1. |
| Conjugation | Un-conjugated |
| Alternate Names | P60; Hop; IEF-SSP-3521; Renal carcinoma antigen NY-REN-11; HEL-S-94n; Hsc70/Hsp90-organizing protein; HOP; STI1L; Transformation-sensitive protein IEF SSP 3521; Stress-induced-phosphoprotein 1; STI1 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse brain | ||||||
| Observed Size | ~ 65 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | STIP1 |
| Gene Full Name | stress-induced phosphoprotein 1 |
| Background | STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009] |
| Function | Mediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB). [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 63 kDa |
Images (2) Click the Picture to Zoom In
-
ARG59264 anti-STIP1 antibody WB image
Western blot: 35 µg of Mouse brain lysate stained with ARG59264 anti-STIP1 antibody at 1:1000 dilution.
-
ARG59264 anti-STIP1 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG59264 anti-STIP1 antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
