ARG63104
anti-STIM1 antibody [CDN3H4]
anti-STIM1 antibody [CDN3H4] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [CDN3H4] recognizes STIM1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Specificity | The clone CDN3H4 reacts with human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CDN3H4 |
| Isotype | IgG1 |
| Target Name | STIM1 |
| Immunogen | Synthetized peptide (C-terminal cytoplasmic part of STIM1). |
| Conjugation | Un-conjugated |
| Alternate Names | GOK; D11S4896E; Stromal interaction molecule 1; STRMK; TAM; TAM1; IMD10 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Application note: Both reducing and non-reducing condition. ICC/IF: Staining technique: Methanol-aceton fixation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: RBL rat basophilic leukemia cell line ICC/IF: HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | STIM1 |
| Gene Full Name | stromal interaction molecule 1 |
| Background | STIM1 (stromal interacting molecule; also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension. |
| Function | Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. [UniProt] |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 77 kDa |
| PTM | Glycosylation is required for cell surface expression. Phosphorylated predominantly on Ser residues. |
Images (2) Click the Picture to Zoom In
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ARG63104 anti-STIM1 antibody [CDN3H4] WB image
Western blot: RBL rat basophilic leukemia cell lysate stained with ARG63104 anti-STIM1 antibody [CDN3H4].
Lane 2 was stained with Isotype mouse IgG1 control. -
ARG63104 anti-STIM1 antibody [CDN3H4] ICC/IF image
Immunofluorescence: methanol-acetone fixed HeLa cells stained with ARG63104 anti-STIM1 antibody [CDN3H4] followed by incubation with Goat anti-mouse IgG1 Alexa Fluor® 598 (red)
Nuclei were stained with DAPI (blue).
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Review for anti-STIM1 antibody [CDN3H4]
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Clone References
STIM1-Ca(2+) signaling is required for the hypertrophic growth of skeletal muscle in mice.
WB / Mouse
STIM1-directed reorganization of microtubules in activated mast cells.
Impaired Orai1-mediated resting Ca2+ entry reduces the cytosolic [Ca2+] and sarcoplasmic reticulum Ca2+ loading in quiescent junctophilin 1 knock-out myotubes.
WB / Human
