ARG51552
anti-STAT5A phospho (Tyr694) antibody
anti-STAT5A phospho (Tyr694) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Gene Regulation antibody; Signaling Transduction antibody
Publication2
Overview
| Product Description | Rabbit Polyclonal antibody recognizes STAT5A phospho (Tyr694) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | STAT5A |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 694 (D-G-Y(p)-V-K) derived from Human STAT5A. |
| Conjugation | Un-conjugated |
| Alternate Names | Signal transducer and activator of transcription 5A; STAT5; MGF |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | STAT5A |
| Gene Full Name | signal transducer and activator of transcription 5A |
| Background | Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. |
| Function | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30214 Phospho STAT5A Antibody Duo (Total, pY694) Related products: STAT5A antibodies; STAT5A Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 91 kDa |
| PTM | Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway. ISGylated. |
Images (2) Click the Picture to Zoom In
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ARG51552 anti-STAT5A phospho (Tyr694) antibody WB image
Western blot: Extracts from K562 cells untreated or treated with G-CSF stained with ARG51552 anti-STAT5A phospho (Tyr694) antibody.
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ARG51552 anti-STAT5A phospho (Tyr694) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51552 anti-STAT5A phospho (Tyr694) antibody (left) or the same antibody preincubated with blocking peptide (right).
Specific References
Effects of di (2-ethylhexyl) phthalate and high-fat diet on lipid metabolism in rats by JAK2/STAT5.
WB / Rat
Mono-2-ethylhexyl phthalate (MEHP) promoted lipid accumulation via JAK2/STAT5 and aggravated oxidative stress in BRL-3A cells.
WB / Rat
