ARG41885
anti-STAT4 phospho (Tyr693) antibody
anti-STAT4 phospho (Tyr693) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes STAT4 phospho (Tyr693) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | STAT4 |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Tyr693 of Human STAT4. |
| Conjugation | Un-conjugated |
| Alternate Names | SLEB11; Signal transducer and activator of transcription 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HepG2 + IL4 | ||||||
| Observed Size | ~ 86 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P42228 Mouse Signal transducer and activator of transcription 4 Swiss-port # Q14765 Human Signal transducer and activator of transcription 4 |
|---|---|
| Gene Symbol | STAT4 |
| Gene Full Name | signal transducer and activator of transcription 4 |
| Background | The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011] |
| Function | Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation. [UniProt] |
| Calculated MW | 86 kDa |
| PTM | Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41885 anti-STAT4 phospho (Tyr693) antibody WB image
Western blot: HepG2 cells were treated by IL4 (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. 25 µg of cell lysate stained with ARG41885 anti-STAT4 phospho (Tyr693) antibody.
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ARG41885 anti-STAT4 phospho (Tyr693) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG41885 anti-STAT4 phospho (Tyr693) antibody.
