ARG41885

anti-STAT4 phospho (Tyr693) antibody

anti-STAT4 phospho (Tyr693) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes STAT4 phospho (Tyr693)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name STAT4
Antigen Species Human
Immunogen Phosphospecific peptide around Tyr693 of Human STAT4.
Conjugation Un-conjugated
Alternate Names SLEB11; Signal transducer and activator of transcription 4

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2 + IL4
Observed Size ~ 86 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20849 Mouse STAT4

GeneID: 6775 Human STAT4

Swiss-port # P42228 Mouse Signal transducer and activator of transcription 4

Swiss-port # Q14765 Human Signal transducer and activator of transcription 4

Gene Symbol STAT4
Gene Full Name signal transducer and activator of transcription 4
Background The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]
Function Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation. [UniProt]
Calculated MW 86 kDa
PTM Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG41885 anti-STAT4 phospho (Tyr693) antibody WB image

    Western blot: HepG2 cells were treated by IL4 (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. 25 µg of cell lysate stained with ARG41885 anti-STAT4 phospho (Tyr693) antibody.

  • ARG41885 anti-STAT4 phospho (Tyr693) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG41885 anti-STAT4 phospho (Tyr693) antibody.