ARG63646
anti-STAP2 / BKS antibody
anti-STAP2 / BKS antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes STAP2 / BKS |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | STAP2 / BKS |
| Antigen Species | Human |
| Immunogen | C-ELQKKLEKRRALEH |
| Conjugation | Un-conjugated |
| Alternate Names | Signal-transducing adaptor protein 2; BRK substrate; STAP-2; Breast tumor kinase substrate; BKS |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UGK3 Human Signal-transducing adaptor protein 2 |
|---|---|
| Background | This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 45 kDa |
| PTM | Phosphorylated on tyrosine. Tyr-250 may be important for interaction with kinases. Phosphorylated by PTK6 at Tyr-250 modulates PTK6-mediated STAT3 activation. Tyr-22 and Tyr-322 appears to be phosphorylated by SRC. |
Images (4) Click the Picture to Zoom In
-
ARG63646 anti-STAP2 / BKS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63646 anti-STAP2 / BKS antibody at 3.75 µg/ml dilution followed by AP-staining.
-
ARG63646 anti-STAP2 / BKS antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63646 anti-STAP2 / BKS antibody at 3.8 µg/ml dilution followed by AP-staining.
-
ARG63646 anti-STAP2 / BKS antibody WB image
Western blot: 35 µg of Human heart lysate (in RIPA buffer) stained with ARG63646 anti-STAP2 / BKS antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
-
ARG63646 anti-STAP2 / BKS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63646 anti-STAP2 / BKS antibody at 3.75 µg/ml dilution followed by AP-staining.
