ARG63289
anti-SSP29 / ANP32B antibody
anti-SSP29 / ANP32B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig
Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SSP29 / ANP32B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SSP29 / ANP32B |
| Antigen Species | Human |
| Immunogen | C-KRKRETDDEGEDD |
| Conjugation | Un-conjugated |
| Alternate Names | Silver-stainable protein SSP29; PHAPI2; APRIL; SSP29; Acidic protein rich in leucines; Acidic leucine-rich nuclear phosphoprotein 32 family member B; Putative HLA-DR-associated protein I-2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANP32B |
| Gene Full Name | acidic (leucine-rich) nuclear phosphoprotein 32 family, member B |
| Function | Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 29 kDa |
| PTM | Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity). |
Images (3) Click the Picture to Zoom In
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ARG63289 anti-SSP29 / ANP32B antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Breast. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63289 anti-SSP29 / ANP32B antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG63289 anti-SSP29 / ANP32B antibody WB image
Western blot: 35 µg of Human tonsil (A), Rat spleen (B) and Pig spleen (C) lysates (in RIPA buffer) stained with ARG63289 anti-SSP29 / ANP32B antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG63289 anti-SSP29 / ANP32B antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG63289 anti-SSP29 / ANP32B antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
