ARG64868

anti-SRD5A2 / 5-alpha reductase 2 antibody

anti-SRD5A2 / 5-alpha reductase 2 antibody for Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes SRD5A2 / 5-alpha reductase 2
Tested Reactivity Hu
Predict Reactivity Dog, Rat, Pig
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SRD5A2 / 5-alpha reductase 2
Antigen Species Human
Immunogen C-KMFEDYPKSRK
Conjugation Un-conjugated
Alternate Names Type II 5-alpha reductase; SR type 2; EC 1.3.1.22; S5AR 2; 5 alpha-SR2; Steroid 5-alpha-reductase 2; 3-oxo-5-alpha-steroid 4-dehydrogenase 2

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6716 Human SRD5A2

Swiss-port # P31213 Human 3-oxo-5-alpha-steroid 4-dehydrogenase 2

Background This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH). [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 28 kDa

Images (1) Click the Picture to Zoom In

  • ARG64868 anti-SRD5A2 / 5-alpha reductase 2 antibody WB image

    Western blot: Human Testis lysate (35 µg protein in RIPA buffer) stained with ARG64868 anti-SRD5A2 / 5-alpha reductase 2 antibody at 1 µg/ml dilution.