ARG41388

anti-SPRY2 / Sprouty 2 antibody

anti-SPRY2 / Sprouty 2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SPRY2 / Sprouty 2
Tested Reactivity Hu, Rat
Tested Application ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SPRY2 / Sprouty 2
Antigen Species Human
Immunogen Synthetic peptide derived from Human SPRY2 / Sprouty 2.
Conjugation Un-conjugated
Alternate Names Protein sprouty homolog 2; hSPRY2; Spry-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 42 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10253 Human SPRY2

Swiss-port # O43597 Human Protein sprouty homolog 2

Gene Symbol SPRY2
Gene Full Name sprouty RTK signaling antagonist 2
Background This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq, Jul 2008]
Function May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton. Cell projection, ruffle membrane. Note=Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor). [UniProt]
Calculated MW 35 kDa
PTM Cleaved at Pro-144 by the prolyl endopeptidase FAP (seprase) activity (in vitro). [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG41388 anti-SPRY2 / Sprouty 2 antibody WB image

    Western blot: HeLa cell lysate stained with ARG41388 anti-SPRY2 / Sprouty 2 antibody.