Overview

Product Description	Rabbit Polyclonal antibody recognizes SPHK1
Tested Reactivity	Hu, Ms
Predict Reactivity	Rat
Tested Application	FACS, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	SPHK1
Antigen Species	Human
Immunogen	Synthetic peptide of Human SPHK1.
Conjugation	Un-conjugated
Alternate Names	SK 1; SPK 1; EC 2.7.1.91; SPHK; Sphingosine kinase 1

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:50 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	Raji
Observed Size	~ 53 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 20698 Mouse SPHK1 GeneID: 8877 Human SPHK1 Swiss-port # Q8CI15 Mouse Sphingosine kinase 1 Swiss-port # Q9NYA1 Human Sphingosine kinase 1
Gene Symbol	SPHK1
Gene Full Name	sphingosine kinase 1
Background	The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Function	Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. [UniProt]
Cellular Localization	Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner. [UniProt]
Calculated MW	43 kDa

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