ARG45284

anti-SPACA3 antibody

anti-SPACA3 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Polyclonal antibody recognizes SPACA3
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SPACA3
Antigen Species Human
Immunogen Recombinant protein containing to human SPACA3.
Conjugation Un-conjugated
Alternate Names SPACA3; Sperm Acrosome Associated 3; SLLP1; LYZL3; LYC3; CT54; Cancer/Testis Antigen 54; ALLP17; Lysozyme-Like Acrosomal Sperm-Specific Secretory Protein ALLP-17; Sperm Protein Reactive With Antisperm Antibodies; Sperm Acrosome Membrane-Associated Protein 3; Sperm Lyzozyme-Like Acrosomal Protein 1; Sperm Protein Reactive With ASA; Sperm Lysozyme-Like Protein 1; Lysozyme-Like Protein 3; Lysozyme-Like Sperm-Specific Secretory Protein ALLP17; Lysozyme C; SPRASA; LYZC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 40 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 124912 Human SPACA3

GeneID: 75622 Mouse SPACA3

Swiss-port # Q8IXA5 Human Sperm acrosome membrane-associated protein 3

Swiss-port # Q9D9X8 Mouse Sperm acrosome membrane-associated protein 3

Gene Symbol SPACA3
Gene Full Name Sperm Acrosome Associated 3 
Background The protein encoded by this gene is a sperm surface protein that may be involved in adhesion to the egg prior to fertilization. While the encoded protein has significant similarity to lysozyme at the amino acid level, it has no detectable bacteriocidal activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
Function Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro. [UniProt]
Cellular Localization Cytoplasmic vesicle; Membrane; Secreted. [UniProt]
Calculated MW 23 kDa
PTM Disulfide bond. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45284 anti-SPACA3 antibody ICC/IF image

    Immunofluorescence: CACO-2 stained with ARG45284 anti-SPACA3 antibody at 5 ug/ml dilution.

  • ARG45284 anti-SPACA3 antibody WB image

    Western blot: 293T and Daudi stained with ARG45284 anti-SPACA3 antibody at 0.5 μg/ml dilution.

  • ARG45284 anti-SPACA3 antibody FACS image

    Flow Cytometry: Daudi stained with ARG45284 anti-SPACA3 antibody at 1 µg/10^6 cells dilution.

  • ARG45284 anti-SPACA3 antibody WB image

    Western blot: C6 stained with ARG45284 anti-SPACA3 antibody at 0.5 μg/ml dilution.

  • ARG45284 anti-SPACA3 antibody WB image

    Western blot: NIH/3T3 and RAW264.7 stained with ARG45284 anti-SPACA3 antibody at 0.5 μg/ml dilution.