ARG63426
anti-SPA1 / SIPA1 antibody
anti-SPA1 / SIPA1 antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SPA1 / SIPA1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | Both variants represent identical protein (NP_694985.28 and NP_006738.3). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SPA1 / SIPA1 |
| Antigen Species | Human |
| Immunogen | C-ASKQLGSPTADLA |
| Conjugation | Un-conjugated |
| Alternate Names | GTPase-activating protein Spa-1; p130 SPA-1; SPA1; Signal-induced proliferation-associated protein 1; Sipa-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96FS4 Human Signal-induced proliferation-associated protein 1 |
|---|---|
| Background | The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 112 kDa |
Images (1) Click the Picture to Zoom In
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ARG63426 anti-SPA1 / SIPA1 antibody WB image
Western Blot: Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63426 anti-SPA1 / SIPA1 antibody at 1 µg/ml dilution.
