ARG54002
anti-SOX2 antibody
anti-SOX2 antibody for Flow cytometry,Western blot and Human,Mouse
Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes SOX2 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | SOX2 |
| Antigen Species | Mouse |
| Immunogen | Purified recombinant mouse Sox2 protein fragments expressed in E.coli |
| Conjugation | Un-conjugated |
| Alternate Names | Transcription factor SOX-2; MCOPS3; ANOP3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | Lot dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Sox2 |
| Gene Full Name | SRY (sex determining region Y)-box 2 |
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 34 kDa |
| PTM | Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. |
Images (3) Click the Picture to Zoom In
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ARG54002 anti-SOX2 antibody WB image
Western blot: 100 µg of whole cell lysates from shSOX2 #1, shSOX2 #2 or control transfected 5637 cells (Human bladder cancer cell line) stained with ARG54002 anti-SOX2 antibody at 1:1000 dilution.
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ARG54002 anti-Sox2 antibody FACS image
Flow Cytometry: F9 cells stained with ARG54002 anti-Sox2 antibody at 1:100 dilution (green), followed by FITC-conjugated goat mouse IgG. Isotype control, normal mouse IgG (black).
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ARG54002 anti-Sox2 antibody WB image
Western blot: Mouse F9 cell lysates stained with ARG54002 anti-Sox2 antibody at 1:1000 dilution.
Customer's Feedback
Excellent
Review for anti-SOX2 antibody
Application：WB
Sample：5637 (Human bladder cancer cell line), knockdown SOX2.
Sample Loading Amount：100 µg / whole cell lysate
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
