ARG41329
anti-SOX15 antibody
anti-SOX15 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SOX15 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SOX15 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to M1-L233 of Human SOX15. |
| Conjugation | Un-conjugated |
| Alternate Names | SOX20; SOX27; SOX26; Protein SOX-20; Protein SOX-15; Protein SOX-12 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 29 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SOX15 |
| Gene Full Name | SRY (sex determining region Y)-box 15 |
| Background | This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. [provided by RefSeq, Jul 2008] |
| Function | Binds to the 5'-AACAAT-3' sequence. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 25 kDa |
Images (3) Click the Picture to Zoom In
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ARG41329 anti-SOX15 antibody ICC/IF image
Immunofluorescence: A431 cells stained with ARG41329 anti-SOX15 antibody.
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ARG41329 anti-SOX15 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41329 anti-SOX15 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41329 anti-SOX15 antibody WB image
Western blot: 50 µg of samples under reducing conditions. A431, U-87MG, HEK293, K562, U2OS, Rat thymus and Mouse SP20 whole cell lysates stained with ARG41329 anti-SOX15 antibody at 0.5 µg/ml dilution, overnight at 4°C.
