ARG63100
anti-SOS1 antibody [SOS-01]
anti-SOS1 antibody [SOS-01] for ICC/IF,Western blot and Human,Mouse
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [SOS-01] recognizes SOS1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Specificity | The clone SOS-01 reacts with human SOS1, an ubiquitously expressed 150 kDa intracellular protein. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SOS-01 |
| Isotype | IgG1 |
| Target Name | SOS1 |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human SOS1 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | GGF1; GF1; SOS-1; GINGF; Son of sevenless homolog 1; HGF; NS4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Under reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SOS1 |
| Gene Full Name | son of sevenless homolog 1 (Drosophila) |
| Background | The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex. |
| Function | Promotes the exchange of Ras-bound GDP by GTP. Catalytic component of a trimeric complex that participates in transduction of signals from Ras to Rac by promoting the Rac-specific guanine nucleotide exchange factor (GEF) activity (By similarity). [UniProt] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 152 kDa |
| PTM | Phosphorylation at Ser-1134 and Ser-1161 by RPS6KA3 create YWHAB and YWHAE binding sites and which contribute to the negative regulation of EGF-induced MAPK1/3 phosphorylation. |
Images (3) Click the Picture to Zoom In
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ARG63100 anti-SOS1 antibody [SOS-01] ICC/IF image
Immunofluorescence: Human primary fibroblasts stained with ARG63100 anti-SOS1 antibody [SOS-01] (green). Actin cytoskeleton was stained with phalloidin (red) and cell nuclei stained with DAPI (blue).
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ARG63100 anti-SOS1 antibody [SOS-01] WB image
Western blot: 1) K562 and 2) Raji cell lysates stained with ARG63100 anti-SOS1 antibody [SOS-01].
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ARG63100 anti-SOS1 antibody [SOS-01] ICC/IF image
Immunofluorescence: Murine transformed fibroblasts stained with ARG63100 anti-SOS1 antibody [SOS-01] (red). Actin cytoskeleton was stained with phalloidin (green) and cell nuclei stained with DAPI (blue).
