ARG54987
anti-SOD2 antibody
anti-SOD2 antibody for Flow cytometry,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SOD2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SOD2 |
| Antigen Species | Human |
| Immunogen | synthetic peptide Human recombinant SD protein. |
| Conjugation | Un-conjugated |
| Alternate Names | MNSOD; Superoxide dismutase [Mn], mitochondrial; IPOB; EC 1.15.1.1; MVCD6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04179 Human Superoxide dismutase [Mn], mitochondrial |
|---|---|
| Gene Symbol | SOD2 |
| Gene Full Name | superoxide dismutase 2, mitochondrial |
| Background | This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Function | Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. [UniProt] |
| Cellular Localization | Mitochondrion matrix. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 25 kDa |
| PTM | Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG54987 anti-SOD2 antibody WB image
Western blot: 35 µg of K562 cell lysate stained with ARG54987 anti-SOD2 antibody.
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ARG54987 anti-SOD2 antibody FACS image
Flow Cytometry: NCI-H460 cells stained with ARG54987 anti-SOD2 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
