ARG55609

anti-SNX3 antibody

anti-SNX3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes SNX3
Tested Reactivity Hu, Ms
Predict Reactivity Bov, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SNX3
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 78-105 (Center) of Human SNX3.
Conjugation Un-conjugated
Alternate Names Protein SDP3; SDP3; Sorting nexin-3; Grd19; MCOPS8

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:25
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control THP-1

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8724 Human SNX3

Swiss-port # O60493 Human Sorting nexin-3

Gene Symbol SNX3
Gene Full Name sorting nexin 3
Background This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like most family members. This protein interacts with phosphatidylinositol-3-phosphate, and is involved in protein trafficking. A pseudogene of this gene is present on the sex chromosomes. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
Function Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Also can bind phosphatidylinositol 4-phosphate (PtdIns(P4)), phosphatidylinositol 5-phosphate (PtdIns(P5)) and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2) (By similarity). Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex (CSC/VPS). May in part act as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G (By similarity). Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes. Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor TFRC presumably by delivering the tranferrin:transferrin receptor complex to recycling endosomes; the function may involve the CSC retromer subcomplex (By similarity). In the case of Salmonella enterica infection plays arole in maturation of the Salmonella-containing vacuole (SCV) and promotes recruitment of LAMP1 to SCVs. [UniProt]
Cellular Localization Cytoplasmic vesicle, Endosome
Research Area Signaling Transduction antibody
Calculated MW 19 kDa
PTM Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP10 (By similarity).

Images (2) Click the Picture to Zoom In

  • ARG55609 anti-SNX3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kideny tissue stained with ARG55609 anti-SNX3 antibody at 1:25 dilution.

  • ARG55609 anti-SNX3 antibody WB image

    Western blot: 35 µg of THP-1 cell lysate stained with ARG55609 anti-SNX3 antibody at 1:1000 dilution.