ARG52424
anti-SNAP25 phospho (Ser187) antibody
anti-SNAP25 phospho (Ser187) antibody for Western blot and Rat
Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SNAP25 phospho (Ser187) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Gpig, NHuPrm, Zfsh |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SNAP25 |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser187 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Super protein; Synaptosomal-associated 25 kDa protein; bA416N4.2; RIC4; SUP; dJ1068F16.2; SNAP; RIC-4; CMS18; SEC9; SNAP-25; Synaptosomal-associated protein 25 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~25k SNAP25 protein phosphorylated at Ser187 in Western blots. Immunolabeling is completely blocked by blocked λ-Ptase. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SNAP25 |
| Gene Full Name | synaptosomal-associated protein 25 |
| Background | SNAP25 (Synaptosomal associated protein of 25 kDa) is a presynaptic plasma membrane protein that is widely distributed throughout the brain and involved in the regulation of neurotransmitter release. Decreased levels of SNAP25 have been found in the brains of patients with Down Syndrome and Alzheimer’s Disease (Greber et al.,1999). In addition, a significant reduction in the hippocampal expression of SNAP25 has also been found in patients with Schizophrenia (Fatemi et al., 2001). Increasing evidence suggests that SNAP-25 also modulates various ion channels, including voltage gated calcium channels (VGCCs) (Pozzi el al., 2008). Activation of PKC results in the phosphorylation of SNAP-25 on ser187 (Shu et al., 2008). Phosphorylation of SNAP25 on ser187 is believed to cause inhibition of VGCC (Pozzi el al., 2008). Since ser187 phosphorylation is transiently induced by neuronal activity, SNAP25 creates a negative feedback mechanism for controlling neuronal excitability (Pozzi el al., 2008). |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 23 kDa |
| PTM | Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity). |
Images (1) Click the Picture to Zoom In
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ARG52424 anti-SNAP25 phospho (Ser187) antibody WB image
Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~25k SNAP25 phosphorylated at Ser 187 (Control) by using ARG52424 anti-SNAP25 phospho (Ser187) antibody.
Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (λ-Ptase, 400 units/100ul lysate for 30 min).
