ARG63747
anti-SNAP25 antibody
anti-SNAP25 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SNAP25 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms NP_003072 and NP_570824. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SNAP25 |
| Antigen Species | Human |
| Immunogen | C-DEANQRATKMLGSG |
| Conjugation | Un-conjugated |
| Alternate Names | Super protein; Synaptosomal-associated 25 kDa protein; bA416N4.2; RIC4; SUP; dJ1068F16.2; SNAP; RIC-4; CMS18; SEC9; SNAP-25; Synaptosomal-associated protein 25 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: SNAP25 antibodies; Anti-Goat IgG secondary antibodies; Related news: Neuronal Development Marker |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 23 kDa |
| PTM | Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG63747 anti-SNAP25 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63747 anti-SNAP25 antibody at 1 µg/ml dilution followed by HRP-staining.
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ARG63747 anti-SNAP25 antibody WB image
Western blot: 35 µg of Human Cerebellum (A), Mouse brain (B) and Rat brain (C) lysates (in RIPA buffer) stained with ARG63747 anti-SNAP25 antibody at 0.03 µg/ml (A, B) and 0.05 µg/ml (C) dilutions and incubated at RT for 1 hour.
