ARG59483

anti-SMYD3 antibody

anti-SMYD3 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SMYD3
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SMYD3
Antigen Species Human
Immunogen Synthetic peptide of Human SMYD3.
Conjugation Un-conjugated
Alternate Names KMT3E; ZNFN3A1; ZMYND1; bA74P14.1; EC 2.1.1.43; SET and MYND domain-containing protein 3; Histone-lysine N-methyltransferase SMYD3; Zinc finger MYND domain-containing protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:30
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 42 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64754 Human SMYD3

GeneID: 69726 Mouse SMYD3

Swiss-port # Q9CWR2 Mouse Histone-lysine N-methyltransferase SMYD3

Swiss-port # Q9H7B4 Human Histone-lysine N-methyltransferase SMYD3

Gene Symbol SMYD3
Gene Full Name SET and MYND domain containing 3
Background This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Function Histone methyltransferase. Specifically methylates 'Lys-4' and 'Lys-5' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M. [UniProt]
Calculated MW 49 kDa

Images (1) Click the Picture to Zoom In

  • ARG59483 anti-SMYD3 antibody WB image

    Western blot: HeLa cell lysate stained with ARG59483 anti-SMYD3 antibody.