ARG59530
anti-SMPD1 / Acid Sphingomyelinase antibody
anti-SMPD1 / Acid Sphingomyelinase antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SMPD1 / Acid Sphingomyelinase |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Bov |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SMPD1 / Acid Sphingomyelinase |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 391-419 of Human SMPD1 / Acid Sphingomyelinase. |
| Conjugation | Un-conjugated |
| Alternate Names | aSMase; EC 3.1.4.12; NPD; ASMASE; Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASM |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Human cerebellum |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SMPD1 |
| Gene Full Name | sphingomyelin phosphodiesterase 1, acid lysosomal |
| Background | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010] |
| Function | Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. [UniProt] |
| Cellular Localization | Lysosome. Secreted. [UniProt] |
| Calculated MW | 70 kDa |
Images (3) Click the Picture to Zoom In
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ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human testis stained with ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody.
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ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody WB image
Western blot: 20 µg of Human cerebellum lysate stained with ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody at 1:2000 dilution.
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ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody FACS image
Flow Cytometry: K562 cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions and stained with ARG59530 anti-SMPD1 / Acid Sphingomyelinase antibody (right histogram) at 1:25 dilution for 60 min at 37°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
