ARG59526

anti-SMPD1 / Acid Sphingomyelinase antibody

anti-SMPD1 / Acid Sphingomyelinase antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SMPD1 / Acid Sphingomyelinase
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SMPD1 / Acid Sphingomyelinase
Antigen Species Human
Immunogen Recombinant protein of Human SMPD1 / Acid Sphingomyelinase.
Conjugation Un-conjugated
Alternate Names aSMase; EC 3.1.4.12; NPD; ASMASE; Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASM

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control THP-1 + PMA
Observed Size 60 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20597 Mouse SMPD1

GeneID: 6609 Human SMPD1

Swiss-port # P17405 Human Sphingomyelin phosphodiesterase

Swiss-port # Q04519 Mouse Sphingomyelin phosphodiesterase

Gene Symbol SMPD1
Gene Full Name sphingomyelin phosphodiesterase 1, acid lysosomal
Background The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]
Function Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. [UniProt]
Cellular Localization Lysosome. Secreted. [UniProt]
Calculated MW 70 kDa

Images (2) Click the Picture to Zoom In

  • ARG59526 anti-SMPD1 / Acid Sphingomyelinase antibody WB image

    Western blot: THP-1 cells were untreated or treated by PMA (80 nM) for overnight. 25 µg of cell lysates stained with ARG59526 anti-SMPD1 / Acid Sphingomyelinase antibody at 1:1000 dilution.

  • ARG59526 anti-SMPD1 / Acid Sphingomyelinase antibody WB image

    Western blot: 25 µg of Rat liver, Mouse liver and HepG2 cell lysates stained with ARG59526 anti-SMPD1 / Acid Sphingomyelinase antibody at 1:1000 dilution.