ARG63416
anti-SM22 alpha / Transgelin antibody
anti-SM22 alpha / Transgelin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody
Publication1
Overview
| Product Description | Goat Polyclonal antibody recognizes SM22 Alpha / Transgelin |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | NP_003177.2 and NP_001001522.1 represent identical protein. The transgelin protein is found in fibroblasts and smooth muscle and is known as a transformation or shape change protein that is involved with actin cross-linking. Transgelin is one of the earliest markers of differentiated smooth muscle cells and recent evidence suggests that transgelin acts as a tumour suppressor. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SM22 alpha / Transgelin |
| Antigen Species | Human |
| Immunogen | C-MTGYGRPRQIIS |
| Conjugation | Un-conjugated |
| Alternate Names | SM22-alpha; 22 kDa actin-binding protein; Smooth muscle protein 22-alpha; Protein WS3-10; Transgelin; WS3-10; TAGLN1; SMCC; SM22 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Highlight | Related Antibody Duos and Panels: ARG30293 Smooth Muscle Marker Related products: SM22 alpha antibodies; SM22 alpha Duos / Panels; Anti-Goat IgG secondary antibodies; Related news: Besides tumor suppression, what’s p53 busy for during embryogenesis? |
| Research Area | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Smooth Muscle Marker antibody |
| Calculated MW | 23 kDa |
Images (2) Click the Picture to Zoom In
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ARG63416 anti-SM22 alpha / Transgelin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63416 anti-SM22 alpha / Transgelin antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63416 anti-SM22 alpha / Transgelin antibody WB image
Western blot: 35 µg of Human testis lysate (in RIPA buffer) stained with ARG63416 anti-SM22 alpha / Transgelin antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
Customer's Feedback
Good
Review for anti-SM22 alpha / Transgelin antibody
Application：WB
Sample：1) Mouse heart, 2) Rat heart
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-SM22 alpha / Transgelin antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Ulinastatin Inhibits the Proliferation, Invasion and Phenotypic Switching of PDGF-BB-Induced VSMCs via Akt/eNOS/NO/cGMP Signaling Pathway.
WB / Human
