ARG43687

anti-SLC4A1 / Band 3 antibody

Datasheet

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100 μl

389.00

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Datasheet

Overview

Product Description	Rabbit Polyclonal antibody recognizes SLC4A1 / Band 3
Tested Reactivity	Hu, Ms
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	SLC4A1 / Band 3
Antigen Species	Human
Immunogen	Synthetic peptide corresponding to Human SLC4A1 / Band 3.
Conjugation	Un-conjugated
Protein Full Name	Band 3 anion transport protein
Alternate Names	AE1; FR; WD; Anion exchanger 1; DI; RTA1A; Solute carrier family 4 member 1; EPB3; AE 1; SW; WD1; CD233; BND3; CD antigen CD233; WR; EMPB3; Anion exchange protein 1; Band 3 anion transport protein

Application Instructions

Application Suggestion

Tested Application	Dilution
WB	1:500 - 1:1000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Observed Size

~ 100 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative	0.01% Sodium azide
Stabilizer	40% Glycerol and 0.05% BSA
Concentration	Batch dependent
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 20533 Mouse SLC4A1 GeneID: 6521 Human SLC4A1 Swiss-port # P02730 Human Band 3 anion transport protein Swiss-port # P04919 Mouse Band 3 anion transport protein
Gene Symbol	SLC4A1
Gene Full Name	solute carrier family 4 (anion exchanger), member 1 (Diego blood group)
Background	The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis. [provided by RefSeq, Jul 2008]
Function	Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine. [UniProt]
Cellular Localization	Cell membrane; Membrane [UniProt]
Calculated MW	102 kDa
PTM	Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation. Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation. N-glycosylated. [UniProt]