ARG41750
anti-SLC27A2 / FATP2 antibody
anti-SLC27A2 / FATP2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SLC27A2 / FATP2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SLC27A2 / FATP2 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 30-200 of Human SLC27A2 / FATP2 (NP_001153101.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Fatty-acid-coenzyme A ligase, very long-chain 1; Long-chain-fatty-acid--CoA ligase; FATP-2; EC 6.2.1.-; HsT17226; VLCS; hFACVL1; Very long-chain acyl-CoA synthetase; Fatty acid transport protein 2; VLACS; FATP2; Very long-chain-fatty-acid-CoA ligase; ACSVL1; EC 6.2.1.3; THCA-CoA ligase; FACVL1; Solute carrier family 27 member 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HepG2 | ||||||||
| Observed Size | ~ 70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SLC27A2 |
| Gene Full Name | solute carrier family 27 (fatty acid transporter), member 2 |
| Background | The protein encoded by this gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme activates long-chain, branched-chain and very-long-chain fatty acids containing 22 or more carbons to their CoA derivatives. It is expressed primarily in liver and kidney, and is present in both endoplasmic reticulum and peroxisomes, but not in mitochondria. Its decreased peroxisomal enzyme activity is in part responsible for the biochemical pathology in X-linked adrenoleukodystrophy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009] |
| Function | Acyl-CoA synthetase probably involved in bile acid metabolism. Proposed to activate C27 precurors of bile acids to their CoA thioesters derivatives before side chain cleavage via peroxisomal beta-oxidation occurs. In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. Does not utilize C24 bile acids as substrates. In vitro, also activates long- and branched-chain fatty acids and may have additional roles in fatty acid metabolism. May be involved in translocation of long-chain fatty acids (LFCA) across membranes (By similarity). [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Peroxisome membrane; Multi-pass membrane protein. Note=Peripheral membrane associated with the lumenal side of peroxisomes. [UniProt] |
| Calculated MW | 70 kDa |
Images (2) Click the Picture to Zoom In
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ARG41750 anti-SLC27A2 / FATP2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG41750 anti-SLC27A2 / FATP2 antibody at 1:100 dilution.
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ARG41750 anti-SLC27A2 / FATP2 antibody WB image
Western blot: 25 µg of HepG2 cell lysate stained with ARG41750 anti-SLC27A2 / FATP2 antibody.
