ARG59227
anti-SKAP55 antibody
anti-SKAP55 antibody for IHC-Formalin-fixed paraffin-embedded sections and Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SKAP55 |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Hm |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SKAP55 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 163-189 of Human SKAP55. (RMAPHLRRDSKKESCFELTSQDRRSYE) |
| Conjugation | Un-conjugated |
| Alternate Names | Src family-associated phosphoprotein 1; SCAP1; Src kinase-associated phosphoprotein 1; SKAP55; pp55; Src kinase-associated phosphoprotein of 55 kDa; HEL-S-81p; SKAP-55 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q4V7G1 Rat Src kinase-associated phosphoprotein 1 |
|---|---|
| Gene Symbol | SKAP1 |
| Gene Full Name | src kinase associated phosphoprotein 1 |
| Background | This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. [provided by RefSeq, Jul 2008] |
| Function | Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Cell membrane. Note=Upon T-cell stimulation, translocates to lipid rafts at the cell membrane. [UniProt] |
| Calculated MW | 41 kDa |
| PTM | Phosphorylated on tyrosines. Phosphorylation by FYN on Tyr-271 is required for GRB2 interaction. Phosphorylation by FYN on Tyr-295 abolishes interaction with FYB. Tyr-232 is dephosphorylated by PTPRC (Probable). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG59227 anti-SKAP55 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat spleen stained with ARG59227 anti-SKAP55 antibody at 1 µg/ml dilution.
