ARG63826
anti-SIRT3 antibody
anti-SIRT3 antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SIRT3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SIRT3 |
| Antigen Species | Human |
| Immunogen | C-QRETGKLDGPDK |
| Conjugation | Un-conjugated |
| Alternate Names | NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2L3; hSIRT3; EC 3.5.1.-; Regulatory protein SIR2 homolog 3; SIR2-like protein 3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NTG7 Human NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
|---|---|
| Background | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 44 kDa |
| PTM | Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. |
Images (2) Click the Picture to Zoom In
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ARG63826 anti-SIRT3 antibody WB image
Western Blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG63826 anti-SIRT3 antibody at 0.2 µg/ml dilution.
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ARG63826 anti-SIRT3 antibody WB image
Western Blot: 1). Mock transfection; 2) SIRT3 (RC200190) expressing plasmid transfected HEK293 cell lysate standed with ARG63826 anti-SIRT3 antibody
