ARG54659
anti-SIRT3 antibody
anti-SIRT3 antibody for ELISA,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody
Overview
| Product Description | Chicken Polyclonal antibody recognizes SIRT3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, WB |
| Host | Chicken |
| Clonality | Polyclonal |
| Isotype | IgY |
| Target Name | SIRT3 |
| Immunogen | Synthetic peptide (17 aa) within aa. 180-230 of Human SIRT3. |
| Conjugation | Un-conjugated |
| Alternate Names | NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2L3; hSIRT3; EC 3.5.1.-; Regulatory protein SIR2 homolog 3; SIR2-like protein 3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse Heart Tissue Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8R104 Mouse NAD-dependent protein deacetylase sirtuin-3 Swiss-port # Q9NTG7 Human NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
|---|---|
| Gene Symbol | SIRT3 |
| Gene Full Name | sirtuin 3 |
| Background | SIRT3 Antibody: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence, lifespan extension and several age-related diseases. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. | |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 44 kDa |
| PTM | Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. |
Images (1) Click the Picture to Zoom In
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ARG54659 anti-SIRT3 antibody WB image
Western blot: mouse heart tissue lysate stained with ARG54659 anti-SIRT3 antibody at (A) 1 and (B) 2 μg/ml.
