ARG64063

anti-SIRT1 antibody

anti-SIRT1 antibody for Western blot and Mouse

Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody

Overview

Product Description Goat Polyclonal antibody recognizes SIRT1
Tested Reactivity Ms
Predict Reactivity Hu, Rat, Dog
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name SIRT1
Antigen Species Human
Immunogen C-EITEKPPRTQKE
Conjugation Un-conjugated
Alternate Names 75SirT1; SIR2L1; SIR2alpha; SIR2-like protein 1; EC 3.5.1.-; NAD-dependent protein deacetylase sirtuin-1; SIR2; hSIRT1; Regulatory protein SIR2 homolog 1; hSIR2

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 93759 Mouse SIRT1

Swiss-port # Q923E4 Mouse NAD-dependent protein deacetylase sirtuin-1

Background This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody
Calculated MW 82 kDa
PTM Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53.
Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 activates deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity). Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545.
Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1).
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity.

Images (2) Click the Picture to Zoom In

  • ARG64063 anti-SIRT1 antibody WB image

    Western Blot: Mouse fetal Brain lysate (35 µg protein in RIPA buffer) stained with ARG64063 anti-SIRT1 antibody at 1 µg/ml dilution.

  • ARG64063 anti-SIRT1 antibody WB image

    Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG64063 anti-SIRT1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.