ARG51793
anti-SHP2 phospho (Tyr580) antibody
anti-SHP2 phospho (Tyr580) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SHP2 phospho (Tyr580) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SHP2 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 580 (R-V-Y(p)-E-N) derived from Human SHP-2. |
| Conjugation | Un-conjugated |
| Alternate Names | BPTP3; SHP-2; Protein-tyrosine phosphatase 2C; METCDS; PTP2C; Tyrosine-protein phosphatase non-receptor type 11; PTP-1D; CFC; PTP-2C; JMML; Shp2; Protein-tyrosine phosphatase 1D; NS1; EC 3.1.3.48; SH-PTP2; SH-PTP3; SHP2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PTPN11 |
| Gene Full Name | protein tyrosine phosphatase, non-receptor type 11 |
| Background | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. |
| Function | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity. [UniProt] |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 68 kDa |
| PTM | Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB. |
Images (2) Click the Picture to Zoom In
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ARG51793 anti-SHP2 phospho (Tyr580) antibody WB image
Western blot: Extracts from 293 cells, treated with starve or calf intestinal phosphatase (CIP), stained with ARG51793 anti-SHP2 phospho (Tyr580) antibody.
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ARG51793 anti-SHP2 phospho (Tyr580) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51793 anti-SHP2 phospho (Tyr580) antibody (left) or the same antibody preincubated with blocking peptide (right).
