ARG51791
anti-SHP1 phospho (Tyr536) antibody
anti-SHP1 phospho (Tyr536) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SHP1 phospho (Tyr536) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SHP1 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 536 (S-E-Y(p)-G-N) derived from Human SHP-1. |
| Conjugation | Un-conjugated |
| Alternate Names | HCP; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase SHP-1; SH-PTP1; PTP-1C; HPTP1C; HCPH; Tyrosine-protein phosphatase non-receptor type 6; SHP-1; EC 3.1.3.48; SHP1; SHP-1L; Protein-tyrosine phosphatase 1C |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PTPN6 |
| Gene Full Name | protein tyrosine phosphatase, non-receptor type 6 |
| Background | Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. |
| Function | Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 68 kDa |
| PTM | Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity. |
Images (2) Click the Picture to Zoom In
-
ARG51791 anti-SHP1 phospho (Tyr536) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51791 anti-SHP1 phospho (Tyr536) antibody (left) or the same antibody preincubated with blocking peptide (right).
-
ARG51791 anti-SHP1 phospho (Tyr536) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51791 anti-SHP1 phospho (Tyr536) antibody.
