ARG41736
anti-SHMT2 antibody
anti-SHMT2 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SHMT2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SHMT2 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 265-504 of Human SHMT2 (NP_005403.2). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.1.2.1; SHMT; GLYA; Serine methylase; Glycine hydroxymethyltransferase; HEL-S-51e; Serine hydroxymethyltransferase, mitochondrial |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HeLa | ||||
| Observed Size | ~ 56 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P34897 Human Serine hydroxymethyltransferase, mitochondrial |
|---|---|
| Gene Symbol | SHMT2 |
| Gene Full Name | serine hydroxymethyltransferase 2 (mitochondrial) |
| Background | This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009] |
| Function | Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. [UniProt] |
| Cellular Localization | Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid. Mitochondrion inner membrane. Cytoplasm. Nucleus. Note=Mainly localizes in the mitochondrion. Also found in the cytoplasm and nucleus as part of the BRISC complex (PubMed:24075985). [UniProt] |
| Calculated MW | 56 kDa |
Images (1) Click the Picture to Zoom In
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ARG41736 anti-SHMT2 antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG41736 anti-SHMT2 antibody at 1:1000 dilution.
